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41.
以尾巨桉(DH32-29)幼苗为试验材料,以植株的生长指标(苗高、地径、叶面积)、生物量、根冠比、氮平衡指数(NBI)、叶绿素相对含量(SPAD 值)、丙二醛(MDA)含量和叶绿素荧光参数为指标,通过盆栽试验设置8个10~140 mg·kg-1浓度镉(Cd)盐溶液处理,研究了不同浓度 Cd 胁迫处理下尾巨桉幼苗的形态和生理特性变化规律。结果显示:低浓度(10 mg·kg-1)Cd 处理对尾巨桉幼苗的形态和生理特性无明显影响。当 Cd 浓度大于20 mg·kg-1,随着 Cd浓度的逐渐增大,其对尾巨桉幼苗的伤害程度也逐渐增加,主要表现为:植株生长缓慢、生物量减小、叶绿素相对含量和叶绿素荧光参数(Fv/Fm、φPSⅡ、NP、NBI)均下降,qP 无明显变化, MDA 含量升高。Cd 浓度大于60 mg·kg-1以上时,尾巨桉幼苗生长出现一定程度上的损伤症状和生长停滞现象。结果表明低浓度(≤20 mg·kg-1)Cd处理下尾巨桉幼苗能正常生长,随着浓度的增大,Cd 处理对尾巨桉的形态和生理特性造成显著影响。同时结合部分桉树适生区土壤 Cd 含量调查及相关研究结果认为,尾巨桉具有一定修复 Cd 污染区土壤的潜力。 相似文献
42.
采用解剖观察、组织切片及人工催情等方法,研究芙蓉鲤鲫(Cyprinus capiofurong.♀×Carassius auratus red var.♂)的性腺发育及繁殖性能。结果显示:解剖观察,芙蓉鲤鲫性腺发育异常的个体占83.64%,性腺外观基本正常的个体占16.36%。组织学观察,芙蓉鲤鲫性腺可分三类:两性嵌合体占21.13%,其中以精巢为主的16.90%,以卵巢为主的4.23%,它们性腺发育迟缓,在繁殖季节成熟系数也没有明显变化,性腺多为一侧发育或两侧不对称,两种性腺细胞均不能成熟;雌性个体占40.84%,在生殖季节卵巢可发育到第IV时相,但未见其细胞核发生偏移;雄性个体,占38.03%,在生殖季节只能发育到精子细胞阶段,不能产生成熟的精子。经人工催产,芙蓉鲤鲫少部分个体有发情产卵行为但不能正常受精。结果表明芙蓉鲤鲫为两性败育类型。 相似文献
43.
玉米精播机漏播补偿系统设计 总被引:21,自引:2,他引:21
目前玉米免耕播种机的播种质量仍低于常规播种。本文讨论了微机控制的排种监测及漏播补偿装置,通过机电结合手段解决漏播问题。台架试验表明本装置能够准确发现漏播,并通过采取的措施实现及时补种,补种成功率可达90%以上。 相似文献
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46.
小黑杨抗寒转录因子PnsICE1基因克隆与生物信息学分析 总被引:1,自引:0,他引:1
ICE1基因是能够编码类似MYC的b HLH转录因子,在低温条件下能够特定地结合CBF3基因启动子中的顺式作用元件,以诱导这个基因所能调控的下游基因的表达。本研究以4℃低温处理24 h的小黑杨(Populus simonii×P.nigra)c DNA为模板,克隆ICE1基因,命名为Pns ICE1。其c DNA长1 650 bp,可编码549个氨基酸。利用Phytozome数据库、NCBI网站、Prot Param、Prot Scale、Pfam27.0和MEGA软件对小黑杨ICE1基因进行生物信息学分析,BLAST分析表明,c DNA序列及其推导的氨基酸序列均与毛果杨(P.trichocarpa)和甜杨(P.suaveolens)ICE1存在着较高的同源性,预示所获得的c DNA可能是小黑杨ICE1基因(Pns ICE1)。 相似文献
47.
Lixia Wang Yuan Guan Rongxia Guan Yinghui Li Yansong Ma Zhimin Dong Xian Liu Haiyan Zhang Yueqiang Zhang Zhangxiong Liu Ruzhen Chang Haiming Xu Linhai Li Fanyun Lin Weijiang Luan Zhe Yan Xuecheng Ning Li Zhu Yanhua Cui Rihua Piao Yan Liu Pengying Chen Lijuan Qiu 《Euphytica》2006,151(2):215-223
It is very important to efficiently study and use genetic diversity resources in crop breeding and sustainable agriculture. In this study, different sampling methods and sample sizes were compared in order to optimize the strategies for building a rationally sized core collection of Chinese soybean (Glycine max). The diversity in the core collection captured more than 70% of that in the pre-core collection, no matter what sampling methods were used, at a sampling proportion of 1%. Core collections established with both simple sequence repeat (SSR) marker data and agronomic traits were more representative than those chosen on an independent basis. An optimal sampling method for a soybean core collection was determined, in which strategy ‘S’ (allocating accessions to clusters according to the proportion of square root of the original sample size within each ecotype) was used based on SSR and agronomic data. Curve estimation was used to estimate the allelic richness of the entire Chinese soybean germplasm and a minimum sample size for a core collection, on which a sampling proportion of about 2% was determined to be optimal for a core collection. Further analysis on the core collection with fourteen agronomic traits and allelic constitution at 60 SSR loci suggested that it highly represented the entire collections both on genetic structure and diversity distribution. This core collection would provide an effective platform in proper exploitation of soybean germplasm resources for the study of complex traits and discovering important novel traits for crop genetic development. 相似文献
48.
Saicum Pintasen Chanakan Prom-u-thai Sansanee Jamjod Narit Yimyam Benjavan Rerkasem 《Euphytica》2007,157(1-2):27-34
Crown rust caused by Puccinia coronata Cda. f. sp. avenae Eriks., is a major fungal disease of cultivated oats (Avena sativa L.) in Tunisia. Six landrace oats (MtK2, JT5, BJ35, GT1, ZN3 and JT0) locally collected and previously screened for their relative oat crown rust resistance were used in this experiment. These
accessions were evaluated during a two cropping season in field plots under heavy natural oat crown rust infection. Assessment
criteria to crown rust reactions were AUDPC, latent period, uredinia size and number of uredinia per cm2 of infected leaf area. Principal components analysis showed that the variables were grouped in two components. These two
principal components explained 83.56% of the total variance. The projection of the point-cloud representing the populations
on the plan formed by the principal components ‘Fact 1’ (63.83%), in abscissa, and ‘Fact 2’ (19.73%) in ordinate, permitted
to distribute the accessions in several groups. Three landrace oats (MtK2, JT5, and JT0) showed the lowest value of all the components used. These landrace oats might be good sources of effective and durable resistance
to crown rust. 相似文献
49.
Kayode A. Sanni I. Fawole R. G. Guei D. K. Ojo Eklou A. Somado D. Daniel Tia S. Ayoni Ogunbayo I. Sanchez 《Euphytica》2008,160(3):389-400
The knowledge of agro-morphological diversity and the distribution pattern of variation among conserved accessions could be an invaluable aid in germplasm management and crop improvement strategies. In this study, the geographical pattern of morphological variation of 880 landrace rice (Oryza sativa (L.)) accessions in Côte d’Ivoire was evaluated for 13 agro-morphological characters. Shannon –weaver diversity index indicated an overall mean of 0.47 ± 0.07 in the collection, with the greatest diversity from derived Savanna and north-west (H′ = 0.52 and 0.50) while the west-central had the lowest diversity (H′ = 0.41). Canonical discriminant analysis showed that traits such as panicle length, grain size (weight and length), tillering ability, number of days to heading and maturity were the main discriminatory characteristics. Result of the phenotypic frequency shows that, the landraces from the north and north-western zones were mostly tall, early heading and maturity, compared to those from the west and west-central which were mainly dwarf to medium height, late heading and maturing. This differential distribution of landraces with height, heading and maturity period reflected the distribution pattern of different Oryza sativa landraces in Côte d’Ivoire, which could be useful in germplasm management and breeding programs. 相似文献
50.